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Basic Concepts in Biochemistry A Student's Survival Guide by Hiram Gilbert

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By Hiram Gilbert

"...reviews the main tricky techniques in biochemistry for max comprehension in a brief interval of time...emphasizes a gaining knowledge of of primary thoughts, no longer memorizing evidence, in order that scholars actually understand & can practice the fabric.

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Extra info for Basic Concepts in Biochemistry A Student's Survival Guide

Sample text

Think of it as an arrow. If the top strand sequence is written with the 5Ј end on the left (this is the conventional way), the bottom strand will have a complementary sequence, and the phosphate backbone will run in the opposite direction; the 3Ј end will be on the left. , it always appears on exams). Either of the two strands could be written on top (just rotate the paper by 180°), but if the DNA codes for a protein, the top strand is usually arranged so that it matches the sequence of the RNA that would be made from the DNA (see later).

These genetic rearrangements within the same piece of DNA actually cause DNA to be lost. Once the recombination is done it’s done, and this cell and its offspring are committed to producing one specific light-chain protein. If the antibody made by a specific cell actually recognizes something foreign, the cell is saved and copied; if not, the cell dies. The large number of different antibody-recognition sites is made possible by the random joining of one of the many variable (V) segments to one of the joining segments by recombination (Fig.

Negative regulators (repressors): Turn off transcription when a specific effector protein binds to a specific repressor sequence in the DNA. BG McGraw-Hill: Gilbert, Basic Concepts in Biochemistry, JN 5036 • 50 • Basic Concepts in Biochemistry V segments (100's) D segments (10's) J segments (4) Constant Region First recombination between D and J segments brings these segments together DJ Constant Region Second rearrangement brings randomly selected V, J, and D regions together V-D-J segments joined Figure 5-5 Constant Region Recombination in Immunoglobulin Genes Recombination is used to randomly combine a variable and two joining segments of the immunoglobulin heavy-chain genes.

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