Chemical Engineering

Applied Biocatalysis: From Fundamental Science to Industrial by Lutz Hilterhaus, Andreas Liese, Ulrich Kettling, Garabed

Posted On February 25, 2017 at 3:34 pm by / Comments Off on Applied Biocatalysis: From Fundamental Science to Industrial by Lutz Hilterhaus, Andreas Liese, Ulrich Kettling, Garabed

By Lutz Hilterhaus, Andreas Liese, Ulrich Kettling, Garabed Antranikian

This reference e-book originates from the interdisciplinary study cooperation among academia and undefined. In 3 special components, most recent effects from uncomplicated learn on strong enzymes are defined and taken into context with attainable business purposes. Downstream processing know-how in addition to biocatalytic and biotechnological construction methods from worldwide avid gamers show the large power of biocatalysts. program of "extreme" response stipulations (i.e. unconventional, equivalent to hot temperature, strain, and pH worth) - biocatalysts are generally used inside of a good outlined strategy window - ends up in novel artificial results. either novel enzyme platforms and the unreal routes during which they are often utilized are made obtainable to the reader. furthermore, the complementary cutting edge approach expertise below unconventional stipulations is highlighted by means of most up-to-date examples from biotech undefined.

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These residues act cooperatively in the catalytic mechanism of ester hydrolysis [4]. The lipolytic reaction takes place at the interface between an insoluble substrate phase and the aqueous phase in which the enzyme is dissolved. Lipases are activated by the presence of this emulsion interface, a phenomenon called interfacial activation, which differentiates them from similar esterases. Upon contact with the hydrophobic interface, a lid domain that covers the active site moves away, turning the closed form of the enzyme into an open form, and leaving the active site available for substrate binding and processing.

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Opin. , 23, 751–757. G. and Budisa, N. (2011) Expanding and engineering the genetic code in a single expression experiment. ChemBioChem, 12, 552–555. M. (2012) Different strategies to enhance the activity of lipase catalysts. Catal. Sci. , 2, 1531–1543. , Kondo, A. et al. (2011) Development of an Aspergillus oryzae whole-cell biocatalyst coexpressing triglyceride and partial glyceride lipases for biodiesel production. Bioresour. , 102, 6723–6729. , and Lin, Y. (2013) Combined utilization of lipase-displaying Pichia pastoris whole-cell biocatalysts to improve biodiesel production in cosolvent media.

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